Down Syndrome

Enzyme Lab Emily Hawthorne October 22, 2012 Biology 121 Mr. Swift Purpose: To measure the effect of changes in temperature, pH, and enzyme intentness on reply rates of enzyme catalyzed reaction in a controlled experiment and to explain how environmental factors yarn-dye the rate of enzyme-catalyzed reactions. Materials/ machine: 1 molar Hcl solution (in dropper bottle) 1 molor NaOh soluton (in dropper bottle) 6 probe electron tubes criterion Pipette 10-ml Graduated cylinder 40 ml 3% Hydrogen enthalpy peroxide solution (found in stores) Sraight-edged shave blade Scissors and Forceps (tweezers) Stirring rod Fresh colorful, Apple, and tater Test tube bearers Ice toilet Warm excrete bath Boiling peeing bath Procedures: business off glass A - Observe Normal Catalase Reaction state of affairs 2 ml of the 3% atomic number 1 peroxide solution into a tripping test tube. Using forceps and scissors, cut a subtile stray of colorful and add it to the test tube. Push in into the hydrogen peroxide with a stirring rod. Observe the bubbles. teem forth the liquid into a second test tube. add unneurotic another 2 ml of hydrogen peroxide to the liver-colored remaining in the first test tube.

Part B What Tissues Contain Catalase You will now test for the presence of catalase in tissues other than liver. shopping center 2 ml of the hydrogen peroxide in each of the 3 clean test tubes and then ass each of the three test substances to the tubes. Part C What is the Effect of the Temperature on the Catalase Activity? Put a piece of the liver into the bottom on a clean test tube a! nd cover it with a small summate of peeing. Place this test tube in a boling water bath for 5 minutes. Remove the test tube from the sulphurous water bath, allow it to air cool, then pour fall out the water. amplify 2 ml of hyrdogen peroxide. CAUTION : Use a test-tube holder for hot test tubes. Put equal quantities of liver into 2 clean test tubes and 1 ml H2O2 into an ice bath. Place the other set in...If you want to get a beat essay, order it on our website: OrderEssay.net

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